Anti-Human VEGF Recombinant Antibody Fab Fragment (TAB-012-F(E))

Name: Anti-Human VEGF Recombinant Antibody Fab Fragment (TAB-012-F(E))
SKU: TAB-012-F(E)
Rating: 5 (1 reviews)

CAT#: TAB-012-F(E)

Recombinant monoclonal antibody Fragment Fab to Human VEGF. It is a monoclonal antibody fragment (Fab) derived from the same parent mouse antibody as bevacizumab (bevacizumab). It is much smaller than the parent molecule and has been affinity matured to provide stronger binding to VEGF-A. It is an anti-angiogenic that has been approved to treat the "wet" type of age-related macular degeneration (AMD, also ARMD), a common form of age-related vision loss.

Tested Data Gene Expression

ELISA

Figure 1 Anti-Human VEGF therapeutic Antibody Fab Fragment (TAB-012-F(E)) in ELISA

ELISA analysis of TAB-012-F(E) was performed by coating with VEGF165 Protein, Human / Cynomolgus, Recombinant.

Figure 2 Anti-Human VEGF therapeutic Antibody Fab Fragment (TAB-012-F(E)) in Dot Blot

Dot Blot analysis of TAB-012-F(E) was performed with VEGF165 Protein, Human / Cynomolgus, Recombinant.
TAB-012-F(E) incubation concentration: 1.6ng/μL
The secondary antibody: HRP-Anti Human IgG (H+L)

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Normal Tissue Figure 1 Cerebral cortex

Neuronal cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_7_5.jpg

Normal Tissue Figure 2 Thyroid gland

Glandular cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_1_5.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_8_4.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_5_6.jpg

Normal Tissue Figure 7 Lymph node

Non-germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_8.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000112715-VEGFA

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Specifications

Immunogen
Purified intact human VEGF

Host Species
Mouse

Derivation
Humanized (from mouse)

Type
Fab - G1 - kappa

Specificity
Tested positive against native human antigen

Species Reactivity
Human

Applications
IP, IF, FuncS, FC, Neut, ELISA, IHC

MW
48350 g/mol

Related Disease
Diabetic retinopathy

Product Property

Purity
>95.0% as determined by analysis by SDS-PAGE.

Storage
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.

Applications

Application Notes
The VEGFA antibody has been reported in applications of Activ, Migration, SPR, Dot, H&E staining, IHC, RT-PCR, ELISA.
Migration: An aliquot of resuspended cells (250 μl; ~50,000 cells/well) was placed in the upper well of the ECM plate, and MBM (750 μl) with or without ligand (130 pM human VEGF-A165, 7.1 nM human PLGF-2, or 3.5 nM mouse PLGF-2), was mixed with VEGF Trap, bevacizumab, or ranibizumab (inhibitor concentration range 0.013–13 nM) and placed in the lower well following a 1 h incubation of the mixture at room temperature. The ECM plate was incubated for 18–20 h in a 37°C/5% CO2 incubator to allow cells from the upper well to migrate through the FluoroBlok™ membrane towards the lower well. Following migration, cells attached to the underside of the FluoroBlok™ membrane were stained with 500 μL of a 2 μg/mL solution of the fluorescent dye Calcein AM for 1.5 h in a 37°C/5% CO2 incubator.
IHC: Retinal cross-sections (n = 7–10 eyes/group) were prepared for immunohistochemistry. After drying and rehydration in PBS, sections were blocked in 10–20% appropriate serum with 1% BSA in 0.1% or 0.2% Triton X-100 in PBS. Three retinal sections per eye were used for each staining. RGCs, cholinergic amacrine cells, (active) microglia as well as macroglia, the synaptic ribbon terminals, photoreceptors as well as the VEGF-receptor 2 were investigated using specific antibodies. The primary antibodies were incubated at room temperature overnight. Incubation with corresponding secondary antibodies was performed for 60 min. As a nuclear stain DAPI was added. Negative controls were implemented by using secondary antibodies only.
ELISA: A total of 96 well plates were coated with recombinant human VEGF165 at a concentration of 1.0 μg/mL overnight at 4°C (100 μL/well). After washing three times with PBS containing 0.05% Tween-20, the wells were blocked with 3% BSA/PBS overnight at 4°C (200 μL/well), washed five times with PBS containing 0.05% Tween-20, and stored dry at 4°C for later use.

Target

Alternative Names
Ranibizumab;ranibizumab;347396-82-1;DB01270VEGFA;vascular endothelial growth factor A;vascular endothelial growth factor , VEGF;VEGF A;VPF;vascular permeability factor;VEGF;MVCD1;MGC70609;

Gene ID
7422

UniProt ID
P15692

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(Creative Biolabs Cat# TAB-012-F(E), RRID: AB_3111742)

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-012-F(E).pdf

MSDS

MSDS - TAB-012-F(E).pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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